Composite

Part:BBa_K5217015:Design

Designed by: qing kong   Group: iGEM24_BGI-MammothEdu-East   (2024-09-26)


pINO1_tLSCL_TEF1t


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1197
    Illegal EcoRI site found at 2145
    Illegal PstI site found at 179
    Illegal PstI site found at 1077
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1197
    Illegal EcoRI site found at 2145
    Illegal NheI site found at 1550
    Illegal PstI site found at 179
    Illegal PstI site found at 1077
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1197
    Illegal EcoRI site found at 2145
    Illegal BglII site found at 1606
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1197
    Illegal EcoRI site found at 2145
    Illegal PstI site found at 179
    Illegal PstI site found at 1077
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1197
    Illegal EcoRI site found at 2145
    Illegal PstI site found at 179
    Illegal PstI site found at 1077
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1593


Design Notes

This part was constructed to express terpene compounds in Saccharomyces cerevisiae



Source

plasmid

References